PennVet Imaging Core
The Penn Vet Imaging Core (PVIC) was established to provide access to cutting-edge optical imaging capabilities for the University of Pennsylvania, Children's Hospital, and Wistar Institute biological and biomedical research communities. The PVIC includes three confocal microscopes and a widefield TIRF microscope, each of which is configured for intravital, live cell and fixed specimen imaging. The Core Director and Manager have over 50 years of combined imaging experience and are focused on making this technology accessible to all labs, regardless of prior level of experience.
Leica SP5 upright multiphoton system
Leica SP5-2 inverted confocal FLIM/FRET system
Leica DMI400 with Yokagawa spinning disk confocal system
Leica DMI6000 with total internal reflection fluorescence (TIRF) capability
Nikon E600 widefield fluorescence upright microscope with camera
- Access to and training on Volocity and MetaMorph image analysis software is available, as is remote access to Volocity.
Penn Vet Imaging Core Training
The PVIC provides free training, advice, and hands-on assistance to all core users. For training, consultation, and access, contact:
- Gordon Ruthel, PhD
- Dr. Ruthel has more than 25 years of advanced imaging experience. Prior to joining PVIC, Dr. Ruthel ran an imaging core facility for USAMRIID at Fort Detrick.
- Phone: 215-746-0471
- Bruce Freedman, VMD, PhD
- Dr. Freedman has more than 30 years experience using advanced optical imaging techniques in his research. He has served as PVIC Director since the Core's inception in 2008.
- Phone: 215-573-8212
These instruments and the core provide capability and support for imaging structures in fixed specimens as well as dynamic events in living cells and thick tissues. The Department of Pathobiology maintains this facility and oversees the fee structure for this service center.
Flow Cytometry Satellite
The flow cytometry core housing our two units will be located on the third floor of the Hill Pavilion and jointly managed under the School of Medicine Flow Cytometry and Cell Sorting Resource Laboratory (FCCSRL).
- A BD FACS Canto was recently installed: FACSCanto II Flow Cytometry System is capable of detecting 6 fluorescence colors with dual laser (488nm and 633nm) excitation. The BD FACSCanto II boasts a new fluidic and optical design, which allows for high acquisition speed and low sample carryover. Fully digital electronics make for easier multicolor compensation.
- The BD FACSCalibur is a two-laser (488nm and 635nm), four-color analog analyzer.
Please visit www.med.upenn.edu/bmcrc for information on rates, all locations and training, or call the core lab at 215.898.3528. Training is required before using the facility.
To further inquire about the services offered, training, and the fee structure by the FCCSRL, contact the Technical Director, Hank Pletcher, at 215.898.3528.