My clinical, diagnostic and research expertise is veterinary pathology with special focus on orthopedic and gastrointestinal pathology. Current and previous research projects have included clinical research, translational animal models of human and animal disease, and exploratory "basic science" projects incorporating academic and industrial collaborations.
One focus of my orthopedic research is equine laminitis in collaboration with Penn Vet's Laminitis Research laboratory (Dr. Galantino-Homer). Through correlation of clinical and pathological data with transcriptomic and proteomic data, the goals of the research are 1) to more completely understand the pathogenesis of laminitis and 2) to identify potential early biomarkers and molecular targets of laminitis for disease prevention and early therapeutic intervention. One unique aspect of the research involves the study of associated tissue interactions between epidermal and dermal lamellae, and bone of the distal phalanx.
Another focus of my orthopedic research is translational animal models. In collaboration with Dr. Nicky Mason (Department of Clinical Studies- Philadelphia) a canine model of osteosarcoma is used to investigate T-cell mediated immunotherapy targeting tumor ostoblasts expressing Her2/neu antigen. Another series of projects investigate surgical procedures, biomaterials, and devices utilizing large animal models of human orthopedic disease including swine, sheep, and goats in collaboration with Dr. Thomas Schaer at the New Bolton Center's Comparative Orthopedic Research Laboratory (CORL).
My secondary research focus, equine gastrointestinal pathology, includes collaborations with Dr. Louise Southwood (Large Animal Emergency and Critical Care service) and Dr. Dipti Pitta (Agricultural Systems and Microbial Genomics laboratory) from the Department of Clinical Studies-New Bolton Center. Previous and ongoing investigations pertain to the equine microbiome, spontaneous and chronic recurrent colic, post-ischemic intestinal injury, and post-surgical enteric wound healing.
Johnstone L, Engiles JB, Tewari D, Johnson A Retrospective Evaluation of Horses Diagnosed with Neuroborreliosis on Postmortem Examination: 16 Cases (2004-2015). Journal of Veterinary Internal Medicine epub doi: 10.1111/jvim.14369: , 2016.Mason, Nicola, Josephine Gnanandarajah, Julie Engiles, Falon Gray, Danielle Laughlin, Anita Gaurnier-Hausser, Anu Wallecha, Margie Huebner, and Yvonne Paterson. Immunotherapy with a HER2 targeted Listeria induces HER2-specific immunity and demonstrates potential therapeutic effects in a phase I trial in canine osteosarcoma. Clinical Cancer Research : , 2016.Mason J, Gurda B, Van Wettere A, Engiles J, Wilson J, Richardson D Delivery and evaluation of recombinant adeno-associated viral vectors in the equine distal extremity for the treatment of laminitis. Equine Veterinary Journal epub doi: 10.1111/evj.12547: , 2015.Engiles J B, Galantino-Homer H L, Boston R, McDonald D, Dishowitz M, Hankenson K D Osteopathology in the Equine Distal Phalanx Associated With the Development and Progression of Laminitis. Veterinary pathology : , 2015.Soltero-Rivera M, Engiles J B, Reiter A M, Reetz J, Lewis J R, Sánchez M D Benign and Malignant Proliferative Fibro-osseous and Osseous Lesions of the Oral Cavity of Dogs. Veterinary pathology : , 2015.Setlakwe E L, Sweeney R, Engiles J B, Johnson A L Identification of Bartonella henselae in the liver of a thoroughbred foal with severe suppurative cholangiohepatitis. Journal of veterinary internal medicine / American College of Veterinary Internal Medicine 28: 1341-5, 2014.Glatman Zaretsky, Arielle. Engiles, Julie B. Hunter, Christopher A. Infection-induced changes in hematopoiesis. [Review] Journal of Immunology 192: 27-33, 2014.Tomlinson, J.E., Johnson, A.L., Ross, M.W., Engiles, J.B., Levine, D.G., Wisner, W.A., Sweeney, R.W. Successful detection and removal of a functional parathyroid adenoma in a pony using technetium tc 99m sestamibi scintigraphy Journal of Veterinary Internal Medicine 28: 687-692, 2014.Paek, Matthew. Engiles, Julie B. Mai, Wilfried. Prevalence, association with stifle conditions, and histopathologic characteristics of tibial tuberosity radiolucencies in dogs. Veterinary Radiology & Ultrasound 54: 453-8, 2013.Dishowitz MI, Mutyaba PL, Takacs JD, Barr AM, Engiles JB, Ahn A, Hankenson KD. Systemic inhibition of canonical Notch signaling results in sustained callus inflammation and alters multiple phases of fracture healing PLoS ONE 8: e68726, 2013.